General Cell Based Assays
Dojindo Molecular Technologies Inc GLYCOLYSIS/OXPHOS ASSAY KT,50T
Glycolysis/Oxidative phosphorylation AssayEasy test via plate reader, no need for expensive equipmentAll reagent acquired is included, ready to use kitEasy-to-understand detailed protocol
Indigo Biosciences INDIGO BIOSCIENCES Human FGFR4 Reporter Assay System, 1 x 384-well format, FGFR4/α-Klotho
This reporter assay system uses proprietary human cells expressing Human Fibroblast Growth Factor Receptor 4 and α-Klotho (FGFR4/α-Klotho) to quantify luciferase activity for screening functional interactions.
- Utilizes proprietary human cells engineered for constitutive expression of human fibroblast growth factor receptor 4
- Exploits signal transduction via the Ca+2·calcineurin / NFAT cascade
- Quantifies relative changes in luciferase activity for dose-dependent measure
- All-inclusive cell-based reporter assay system with cryopreserved cells for immediate dispensing
Enzo Life Sciences FLUOFORTE Calcium assay kit (96tests)
Fluo-Forte Calcium Assay Kits detect mobilization of intracellular calcium utilizing a fluorogenic calcium-binding dye optimized for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence. Mechanism of Action The fluorogenic calcium-binding dye is provided to the cells as an acetoxymethyl (AM) ester, which is cell-permeable. Once inside the cells, the dye is hydrolyzed by intracellular esterases, which leads to generation of a cell membrane impermeable negatively charged form. In the absence of calcium, the calcium-binding moiety portion of the probe quenches the fluorescence of the fluorophore portion of the probe by photo-induced electron transfer. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Meso Scale Discovery R-PLEX Human GFAP ASY
The R-PLEX Human GFAP Assay Kit from Meso Scale Discovery measures glial fibrillary acidic protein (GFAP) in human samples using electrochemiluminescence (ECL) technology It offers high sensitivity a wide dynamic range and low sample volume requirements The kit includes a 96-well plate with capture and detection antibodies Read Buffer Sample Diluent and calibrators for standard curve generation It quantifies GFAP a biomarker in astrocytes linked to CNS disorders with a 2 5-hour protocol for high-throughput profiling
Profoldin ProFoldin Lipid Bilayer Permeability Disruption Assay, 100 Assays, 96-Well Plate
This assay kit employs a liposome-encapsulated dye to monitor dye release due to lipid bilayer damage. It screens compounds that disrupt permeability and damage cell membranes, such as pore-forming cytotoxins. Fluorescence is monitored at 580 nm (excitation at 540 nm) in a high-throughput 96-well plate format.
- Assay for screening compound collections.
- Liposomes composed of HSPC: cholesterol: DSPE-PEG2000 (3:1:1 mass ratio).
- Encapsulated dye is rhodamine conjugated with diethylenetriamine, quenched inside the liposome.
- Released dye generates fluorescence in the presence of a color development agent.
- Includes 1.25 mL of liposomal dye, 1.25 mL of color development solution, 7.5 mL of buffer, and 1 mL of DMSO.
- Designed for 100 samples using a 96-well plate.
- Protocol provided for 96-well plate fluorescence plate reader with a total sample volume of 100 μL.
Meso Scale Discovery U-PLEX Rat Leptin ASY 25 PL
The Meso Scale Discovery U-PLEX Rat Leptin Assay Kit is a multiplex immunoassay that measures leptin levels in rat serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with MSD SULFO-TAG calibrators and controls The assay has a lower limit of detection of 1 3 pg/mL and an upper limit of quantification of 10 000 pg/mL for leptin
Meso Scale Discovery R-PLEX Human IL1RL1/ST2 ASY
The R-PLEX Human IL1RL1/ST2 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures IL1RL1/ST2 in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL compound and calibrators and controls to generate a standard curve The assay takes 3 5 hours to complete and requires a Meso Scale Discovery instrument such as SECTOR Imager QuickPlex SQ 120 or QuickPlex SQ 120
Meso Scale Discovery U-PLEX Rat Leptin ASY
The Meso Scale Discovery U-PLEX Rat Leptin Assay Kit is a multiplex immunoassay that measures leptin levels in rat serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with ruthenium calibrators and controls The assay requires a small sample volume and provides results in as little as 3 5 hours With minimal hands-on time this kit allows for the rapid accurate quantification of rat leptin
Meso Scale Discovery U-PLEX Mouse IL-17C ASY
The Meso Scale Discovery U-PLEX Mouse IL-17C Assay Kit is a multiplex immunoassay that enables simultaneous quantification of mouse interleukin 17C (IL-17C) in cell culture supernatants serum plasma and other biological matrices This assay utilizes MSD s proprietary electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The kit includes a 96-well plate pre-coated with capture antibodies detection antibodies and calibrators and controls needed to generate a standard curve and quantify IL-17C concentrations This assay allows for rapid reproducible quantification of IL-17C from mouse samples