General Cell Based Assays
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 2,500/10,000 dp (96-well/384-well). (90-0075LM25)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 25 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Enzo Life Sciences PROTEOSTAT Aggresome detection kit (1x100tests)
Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology. Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer's, Parkinson's, Amyotrophic lateral sclerosis or alcoholic liver disease.The PROTEOSTAT.PROTEOSTAT. . Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection. Contents: PROTEOSTATAggresome detection reagent . Hoechst 33342 Nuclear stain. Proteasome Inhibitor (MG-132). 10X Assay Buffer
Dojindo Molecular Technologies Inc Cellular Senescence Detection Kit (1 plate), detects SA-β-gal with high sensitivity, applicable to both living and fixed cells, for cell sample by fluorescence microscopy or flow cytometry.
SPiDER-βGal is a new reagent to detect β-galactosidase specifically which possesses a high cell-permeability and a high retentivity inside cells. This novel reagent can detect β-galactosidase not only in fixed cells but also in living cells, and also can be applied to quantitative analysis by flow cytometry. Senescence-associated beta-galactosidase (SA-β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Cellular Senescence Detection Kit – SPiDER-βGal is optimized to detect intracellular SA-β-gal. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 500 Assay Points
Cell Biolabs Inc Total Cholesterol Assay Kit (Colorimetric), 192 assays
Cell Biolabs’ Total Cholesterol Assay Kit is a simple colorimetric assay that measures the amount of total cholesterol present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, cholesterol standards and unknown samples. Sample cholesterol concentrations are determined by comparison with a known cholesterol standard. Cholesteryl esters can be quantified by subtracting the free cholesterol values from the total cholesterol value.
Agilent Technologies Seahorse XFe96/XF Pro FluxPak Mini, 6 Sensor Cartridges, 6 Cell Culture Microplates, 500 mL Calibrant Solution, 96 Wells per microplate, Room Temperature Storage (4 to 30 °C)
Seahorse XFe96/XF Pro FluxPak Mini, 6 Sensor Cartridges, 6 Cell Culture Microplates, 500 mL Calibrant Solution, 96 Wells per microplate, Room Temperature Storage (4 to 30 °C)
Eurofins Discoverx cAMP Hunter eXpress GLP1R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0062E2CP2M)
The cAMP Hunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences DPPIV/CD26 Assay kit for biological samples (1x96wells)
Because DPPIV/CD26 protein levels may not accurately reflect the levels of active DPPIV enzyme, it is useful to measure DPPIV activity rather than performing DPPIV immunoassay. The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered .½-volume NBS white microplate (Prod. No. BML-KI571)
Abcam Protease Activity Assay Kit (Fluorometric - Red)
Protease Activity Assay Kit (Fluorometric - Red)
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc Cellular Senescence Detection Kit (3 plate), detects SA-β-gal with high sensitivity, applicable to both living and fixed cells, for cell sample by fluorescence microscopy or flow cytometry.
SPiDER-βGal is a new reagent to detect β-galactosidase specifically which possesses a high cell-permeability and a high retentivity inside cells. This novel reagent can detect β-galactosidase not only in fixed cells but also in living cells, and also can be applied to quantitative analysis by flow cytometry. Senescence-associated beta-galactosidase (SA-β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Cellular Senescence Detection Kit – SPiDER-βGal is optimized to detect intracellular SA-β-gal. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
FUJIFILM BIOSCIENCES INC LabAssay NEFA Kit, 500 tests, 4 μL sample volume, 0.4-1.97 mEq/L assay range, 2-10°C storage
The LabAssay NEFA Kit is an enzymatic colorimetric assay designed for the quantitative determination of non-esterified fatty acids (NEFA). This kit supports research on lipid metabolism and metabolic disorders, providing a precise tool for measuring NEFA concentrations in biological samples.
- Designed for non-esterified fatty acids determination
- Utilizes enzymatic colorimetric detection method
- Suitable for various animal serum/plasma and culture supernatants
- Compatible with samples from mice, rats, humans, cats, and dogs
- Fast reaction time of approximately 10 min
- Provides 500 tests per kit
- Storage condition: 2-10 °C
- Research use only, not for diagnostic procedures
- Includes reagents and standard solution for accurate results