General Cell Based Assays
Apexbio Technology LLC APExBIO Thioredoxin Reductase Colorimetric Assay Kit, 100 assays
This kit offers a rapid method for quantifying thioredoxin reductase (TrxR) activity. It utilizes NADPH as a coenzyme for TrxR to catalyze the reduction of DTNB, producing a yellow TNB that absorbs at 412 nm. A TrxR-specific inhibitor is included to eliminate background interference from other enzymes, ensuring high specificity in measuring TrxR activity.
- Rapid quantification of thioredoxin reductase (TrxR) activity.
- Utilizes NADPH as a coenzyme for enzymatic reactions.
- Catalyzes the reduction of DTNB to TNB, detectable at 412 nm.
- Includes a TrxR-specific inhibitor for high specificity and reduced background interference.
- Reliable for TrxR functional studies.
- Shelf life of 1 year.
- Intended for scientific research use only.
Aat Bioquest Fluo-8, AM | 1mg
Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8 dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8 AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading.
Enzo Life Sciences PROTEOSTAT Aggresome detection kit (1x100tests)
Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology. Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer's, Parkinson's, Amyotrophic lateral sclerosis or alcoholic liver disease.The PROTEOSTAT.PROTEOSTAT. . Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection. Contents: PROTEOSTATAggresome detection reagent . Hoechst 33342 Nuclear stain. Proteasome Inhibitor (MG-132). 10X Assay Buffer
Abcam Hydrogen Peroxide Assay Kit
Hydrogen Peroxide Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc Cytotoxicity LDH Assay Kit-WST (500 TESTS) measures released LDH from dead cells and can be used with and without transferring supernatant.
Lactate dehydrogenase(LDH) is an enzyme that is present in almost all cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals or intercellular signals, LDH is rapidly released from the cell membrane. Thus, the measurement of the amount of released LDH from cells is one of the major methods to assess cell death. Since Dojindo's Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is harmful to cells, it allows the assay to perform in wells containing both viable and damaged cells. Cell Cytotoxicity is evaluated by multiple cell functions. Dojindo provides you useful selection guide. (https://www.dojindo.com/cell-proliferation-cytotoxicity-assay-kits-selection-guide/)
TOPOGEN INC DNA Gyrase/Relaxed DNA Assay Kit
The E. coli DNA Gyrase and Relaxed DNA Assay Kit is designed to provide the investigator quick and specific assays for DNA gyrase. These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work. These kits are also appropriate for the researcher who is interested in large scale screening for novel anti-gyrase active agents. We matched the amount of DNA gyrase enzyme with the amount of relaxed pHOT-1 DNA to get you going and keep you going on the hunt for new actives.